Hoover H73 Vacuum Cleaner Bags, Original, Microfiber, Pure Epa, Anti-odour, Extra Large, Compatible with Hoover Athos Cylinder Vacuum Cleaners, 4 Pieces

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Herz, H. M., Garruss, A. & Shilatifard, A. SET for life: biochemical activities and biological functions of SET domain-containing proteins. Trends Biochem. Sci. 38, 621–639 (2013). Trievel, R. C., Beach, B. M., Dirk, L. M., Houtz, R. L. & Hurley, J. H. Structure and catalytic mechanism of a SET domain protein methyltransferase. Cell 111, 91–103 (2002). Note: Press the Heat Link's button again to turn off heating (press twice forNest Thermostat E). Find your errorKwiatkowski, S. et al. SETD3 protein is the actin-specific histidine N-methyltransferase. Elife 7, e37921 (2018). Baek, J.H.; Lee, J.; Yun, H.S.; Lee, C.W.; Song, J.Y.; Um, H.D.; Park, J.K.; Park, I.C.; Kim, J.S.; Kim, E.H.; et al. Kinesin light chain-4 depletion induces apoptosis of radioresistant cancer cells by mitochondrial dysfunction via calcium ion influx. Cell Death Dis. 2018, 9, 496. [ Google Scholar] [ CrossRef] To generate stable cell line expressing NMD3 with an affinity tag at the C-terminus, the DNA sequence encoding NMD3-TEV-3FLAG was amplified from Human NMD3 cDNA library using PCR. The target sequence was cloned into a lentiviral expression vector pLVX-AcGFP1-N1(ClonTech) using ClonExpress II One Step Cloning Kit (Vazyme). Cell transfection was adapted from a previous work with slight modifications 48. The cells were allowed to grow to 80% confluency and transfected with NMD3-tev-3XFLAG in pLVX-AcGFP1-N1, pMD2.G and psPAX2 (from Dr. Li Yu, Tsinghua University) in mass ratio 2:2:3 using Polyethylenimine (PEI) (Linear MW 25,000, Polysciences.Inc, 23966-1), which was added to the plasmid-DMEM solution drop by drop to reach a PEI to total plasmid mass ratio of 3:1 and mixed thoroughly. 48 h after transfection, medium, containing target lentivirus, was collected to infect new HEK293FT for 48 h with 8 μg/ml polybrene. Selection was performed with 2 μg/ml puromycin (Ameresco, J593-25MG). When selection finished, the adherent cell line was tamed to suspension via reducing concentration of FBS from 10% to 1% gradually in SMM 293-TI, containing 1x glutaMAX, 100 U/ml penicillin and 10 mg/ml streptomycin. Purification of pre-60S NMD3 particles These steps cover the following help codes: N1, N21, N71, N2, N22, N72, N3, N23, N73, N153, N4, N24, N74, N15, N5, N25, N75, N6, N26, N76, N156, N7, N27, N77, N28, N78, N29, N79, N159, N261

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